中文名称：抗-DNA-RNA杂交抗体(S96)(RGK60001)

产品描述

DNA-RNA杂交体是细胞内一种普遍存在的自然现象，尤其是在高转录活性位点，其水平较高。这些杂交体代表了非经典的核酸结构，具备转录调控的功能。研究表明，它们的存在可能导致特定基因座的染色体断裂风险增加。DNA:RNA杂交体的形成，为双链A/B中间构象提供了基础，同时在互补的DNA链上形成了二次单链核酸结合蛋白的结合靶点。这些杂交物对DNA甲基转移酶的活性表现出一定的抗性。此外，DNA:RNA杂交体的形成与多种神经系统疾病相关，例如，DNA:RNA解旋酶senataxin（SETX）的突变与显性青少年肌萎缩侧索硬化症4型和隐性眼动失协调2型有密切联系。

产品详情

产品名称：抗-DNA-RNA杂交抗体(S96)(RGK60001)
货号：RGK60001
表达系统：哺乳动物细胞
种属反应性：通用
宿主：小鼠
同种型：IgG2a,kappa
克隆类型：单克隆
靶标：DNA-RNA杂交体
浓度：0.58 mg/ml
内毒素水平：请联系实验室获取该信息
纯度：95%（通过SDS-PAGE测定）
纯化方式：从细胞培养上清中用Protein A/G纯化
克隆号：S96
应用：ChIP, ChIP-seq, DB, EMSA, FISH, ICC, IF, IP, SPR
状态：液体
保存溶液：0.01M PBS, pH 7.4
稳定性和存储：使用手动解冻冰箱，避免反复冻融；短期存放在4°C（1-2周），长期存放在-20°C可达12个月，在-80°C可长期保存。

参考文献

1. Recognition of RNA by the S96 antibody creates pervasive artifacts when imaging RNA:DNA hybrids PMID:33830170
2. Immunoprecipitation of DNA:RNA Hybrids Using the S96 Antibody PMID:32681514
3. High-resolution, strand-specific R-loop mapping via S96-based DNA-RNA immunoprecipitation and high-throughput sequencing PMID:31053798
4. RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage PMID:29742442
5. The monoclonal S96 antibody exhibits highly variable binding affinities towards different R-loop sequences PMID:28594954
6. Structural basis of R-loop recognition by the S96 monoclonal antibody PMID:35347133
7. Best practices for the visualization, mapping, and manipulation of R-loops PMID:33411340
8. Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging PMID:34232287
9. The Affinity of the S96 Antibody for Double-Stranded RNAs Impacts the Accurate Mapping of R-Loops in Fission Yeast PMID:29289567
10. The sub-nanomolar binding of DNA-RNA hybrids by the single-chain Fv fragment of antibody S96 PMID:23784994
11. Modifications and interactions at the R-loop PMID:32961406
12. BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2 PMID:24896180
13. R-Loop Analysis by Dot-Blot PMID:33554969
14. Amplification-Free and Mix-and-Read Analysis of Multiplexed MicroRNAs on a Single Plasmonic Microbead PMID:34324345
15. New insight into the biology of R-loops PMID:32516653
16. Detection of Telomeric DNA:RNA Hybrids Using TeloDRIP-qPCR PMID:33371452
17. Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation PMID:32105733
18. Complexities due to single-stranded RNA during antibody detection of genomic RNA:DNA hybrids PMID:25890199
19. Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening PMID:34495394

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